Sample isolation procedures
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چکیده
We first identified genes differentially expressed in rodent testis by comparing total testis, (TT; gonads whose tunica was removed by decapsulation) and isolated seminiferous tubules (TU; lacking the interstitial cells) to enriched populations of Sertoli cells (SE), spermatogonia (SG), pachytene spermatocytes (SC) and round spermatids (ST). Chondrocytes (CC) and vascular smooth muscle (SM) samples were included as controls because the human Sertoli cell and spermatogonia populations were not sufficiently pure for reliable analysis. To determine the number of conserved loci showing similar meiotic and post-meiotic expression across species, rodent samples were then compared to enriched human germ cells, tubules and total testis samples. To identify the subset of potentially testis-specific transcripts, somatic non-testicular control tissues were included in the analysis. Finally, among conserved, co-expressed and potentially testis-specific proteins, the group of factors known to be component of protein interaction networks was examined. All cell and tissue samples were pooled and processed in duplicate (see Supplementary Fig. 1).
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تاریخ انتشار 2006